Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells

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  • Additional Information
    • Publication Information:
      Spandidos Publications
    • Publication Date:
      2015
    • Subject Terms:
    • Abstract:
      Introduction The majority of the molecular biology based approaches developed for the experimental therapy of thalassemia and sickle-cell anemia (SCA) are based on the induction of defective globin ([beta]-globin in [...]
      In the treatment of hemoglobinopathies, amending altered hemoglobins and/or globins produced in excess is an important part of therapeutic strategies and the selective inhibition of globin production may be clinically beneficial. Therefore the development of drug-based methods for the selective inhibition of globin accumulation is required. In this study, we employed peptide nucleic acids (PNAs) to alter globin gene expression. The main conclusion of the present study was that PNAs designed to target adult murine [beta]-globin mRNA inhibit hemoglobin accumulation and erythroid differentiation of murine erythroleukemia (MEL) cells with high efficiency and fair selectivity. No major effects were observed on cell proliferation. Our study supports the concept that PNAs may be used to target mRNAs that, similar to globin mRNAs, are expressed at very high levels in differentiating erythroid cells. Our data suggest that PNAs inhibit the excess production of globins involved in the pathophysiology of hemoglobinopathies. Key words: peptide nucleic acids, sickle-cell anemia, [beta]-globin, hemoglobin, erythroid differentiation
    • ISSN:
      1107-3756
    • Accession Number:
      10.3892/ijmm.2014.2005
    • Rights:
      Copyright 2015 Gale, Cengage Learning. All rights reserved.
      COPYRIGHT 2015 Spandidos Publications
    • Accession Number:
      edsgcl.401215589
  • Citations
    • ABNT:
      MONTAGNER, G. et al. Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells. International Journal of Molecular Medicine, [s. l.], n. 1, p. 51, 2015. Disponível em: . Acesso em: 17 set. 2019.
    • AMA:
      Montagner G, Gemmo C, Fabbri E, et al. Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells. International Journal of Molecular Medicine. 2015;(1):51. doi:10.3892/ijmm.2014.2005.
    • APA:
      Montagner, G., Gemmo, C., Fabbri, E., Manicardi, A., Accardo, I., Bianchi, N., … Gambari, R. (2015). Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells. International Journal of Molecular Medicine, (1), 51. https://doi.org/10.3892/ijmm.2014.2005
    • Chicago/Turabian: Author-Date:
      Montagner, Giulia, Chiara Gemmo, Enrica Fabbri, Alex Manicardi, Igea Accardo, Nicoletta Bianchi, Alessia Finotti, et al. 2015. “Peptide Nucleic Acids Targeting [Beta]-Globin MRNAs Selectively Inhibit Hemoglobin Production in Murine Erythroleukemia Cells.” International Journal of Molecular Medicine, no. 1: 51. doi:10.3892/ijmm.2014.2005.
    • Harvard:
      Montagner, G. et al. (2015) ‘Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells’, International Journal of Molecular Medicine, (1), p. 51. doi: 10.3892/ijmm.2014.2005.
    • Harvard: Australian:
      Montagner, G, Gemmo, C, Fabbri, E, Manicardi, A, Accardo, I, Bianchi, N, Finotti, A, Breveglieri, G, Salvatori, F, Borgatti, M, Lampronti, I, Bresciani, A, Altamura, S, Corradini, R & Gambari, R 2015, ‘Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells’, International Journal of Molecular Medicine, no. 1, p. 51, viewed 17 September 2019, .
    • MLA:
      Montagner, Giulia, et al. “Peptide Nucleic Acids Targeting [Beta]-Globin MRNAs Selectively Inhibit Hemoglobin Production in Murine Erythroleukemia Cells.” International Journal of Molecular Medicine, no. 1, 2015, p. 51. EBSCOhost, doi:10.3892/ijmm.2014.2005.
    • Chicago/Turabian: Humanities:
      Montagner, Giulia, Chiara Gemmo, Enrica Fabbri, Alex Manicardi, Igea Accardo, Nicoletta Bianchi, Alessia Finotti, et al. “Peptide Nucleic Acids Targeting [Beta]-Globin MRNAs Selectively Inhibit Hemoglobin Production in Murine Erythroleukemia Cells.” International Journal of Molecular Medicine, no. 1 (2015): 51. doi:10.3892/ijmm.2014.2005.
    • Vancouver/ICMJE:
      Montagner G, Gemmo C, Fabbri E, Manicardi A, Accardo I, Bianchi N, et al. Peptide nucleic acids targeting [beta]-globin mRNAs selectively inhibit hemoglobin production in murine erythroleukemia cells. International Journal of Molecular Medicine [Internet]. 2015 [cited 2019 Sep 17];(1):51. Available from: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsgao&AN=edsgcl.401215589&authtype=sso&custid=s1007886